中科院纳米生物效应与安全性重点实验室
纳米技术论坛 (NS Forum No. 308)
第308期学术报告会通知

题 目：Fluorescence Lifetime Imaging for determination of ion concentrations in living cells and tissue  
报告人：Thomas Gensch, Ph.D, Institute of Complex Systems 4 (ICS-4), Cellular Biophysics Forschungszentrum Juelich (Research Centre Juelich) 
时 间：2018年11月14日（星期三）,下午15:00
地 点：国家纳米科学中心，南楼四层会议室 
主持人：戴陆如 副研究员 

报告摘要:
The intra- and extracellular concentration of a few inorganic ions are very important parameters of cells and cell tissue setting the framework or even the driving force for many important physiological processes. The non-invasive determination of these ion concentrations in living cells and their observation in time is a great help when studying physiology and pathophysiology of cells in the context of both basic science as well as medical science. Here, the usefulness of fluorescence lifetime imaging (FLIM) based on time-correlated single photon counting (TCSPC) implemented on a laser-scanning fluorescence microscope with two-photon excitation is proven by presenting results of studies dedicated to determine the absolute concentration of H+, Na+, K+, Cl- and Ca2+ in living cells, cell compartments and cell tissue.

个人简介： 
Thomas Gensch at Helmholtz Society Research Centre Jülich (Germany) is group leader (Fluorescence Spectroscopy and Microscopy) in the Institute of Complex Systems4 (ICS-4; Cellular Biophysics). After studying physics at Humboldt University Berlin, he obtained his Ph.D in 1996 with Professor Silvia Braslavsky and Prof. Georg Büldt in photoacoustic spectroscopy. After two postdoctoral stays with Prof. Frans De Schryver (Dep. Chem.; KU Leuven, Belgium single molecule spectroscopy) and Prof. Klaas Hellingwerf (Dep. Chem.; UvA, Amsterdam, The Netherlands; protein conformation studied by static and dynamic structural and spectroscopic methods) he started in 2000 at ISB-1 (now ICS-4, head: Prof. Benjamin Kaupp; current head: Prof. Christoph Fahlke), where he shifted his interest to advanced fluorescence microscopy methods (e.g. Forster Resonance Energy Transfer, Fluorescence Lifetime Imaging, Single Molecule Localization Microscopy, confocal, wide-field, two-photon and spinning disc confocalfluorescence microscopy). His group modified, adapted and successfully applied all these techniques to a diverse set of biological systems including bacteria,plant cells and tissue, mammalian cell culture cell as well as primary mammalian cells and cell tissues, e.g. from brain, blood vessels, kidney, heart and the nervous system but also non-biological materials like dielectric oxides. FLIM and its application for determination of absolute values of cell parameters in living systems has been a speciality ever since.