题目: Highly Sensitive and Selective DNA-Based Detection of Mercury(II) with α-Hemolysin Nanopore
作者：Shuang Wen,†,‡ Tao Zeng,§,‡ Lei Liu,† Kai Zhao,† Yuliang Zhao,*,†,§ Xianjin Liu,|| and Hai-Chen Wu*,†
单位：
†Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
§National Center for Nanoscience and Technology of China, Beijing 100190, China
||Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Lab of Agro-Food Safety and Quality, Ministry of Agriculture, Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
摘要：
The duplex formation mediated by Hg2+ in a properly designed ssDNA generates a stable hairpin structure, which greatly alters the translocation profile of the ssDNA through α-hemolysin nanopore. From the 2D-events contour plot, the presence of Hg2+ can be confirmed in as little as 30 min at ∼7 nM or higher. The sensor is highly selective to Hg2+, without interference from other metal ions. It can be fabricated from readily available materials, without the processes of synthesis, purification, probe-making, and so forth. This sensing strategy opens new possibilities for detecting many types of analytes which have specific interactions with DNA molecules.
文章发表信息：J. Am. Chem. Soc., 2011, 133 (45), pp 18312–18317
文章网址链接：http://pubs.acs.org/doi/abs/10.1021/ja206983z
